Screening of Amorpha fruticosa and Ailanthus altissima extracts for genotoxicity/antigenotoxicity, mutagenicity/antimutagenicity and carcinogenicity/anticarcinogenicity

The aim of the present study was to evaluate potential genotoxic/antigenotoxic, mutagenic/antimutagenic, and carcinogenic/anticarcinogenic effect Amorpha fruticosa (AF) fruit, Ailanthus altissima bark hexane (AAEH) methanol (AAEM) extracts on a model system Saccharomyces cerevisiae. Plants were identified extracted by Ekaterina Kozuharova. Three concentrations each extract tested – 10, 100 1000 µg/ml. In vitro pro-oxidant/antioxidant activities evaluated DPPH DNA topology assay. mutagenic/antimutagenic effects revealed in vivo by: Zimmermman’s test cerevisiae diploid strain D7ts1, Ty1 retrotransposition S. haploid 551. Zeocin used as positive control. Based antioxidant activity could be arranged follows: AF>AAEM>AAEH. AAEH possessed moderate oxidative potential. No genotoxic mutagenic capacity obtained for tested. levels total aberrants, convertants revertants comparable with control ones. induced treatment. Further, well-expressed antigenotoxic, antimutagenic anticarcinogenic activity. Significant reduction reverse point mutations obtained. Only AF found reduce zeocin-induced mitotic gene conversion. three did not possess any genotoxic, carcinogenic their protective activity, they can AF>AAEM>AAEH which corresponds well phytochemical composition. Further experiments provide more detailed information concerning mode action extracts, main constituents.